NSC95397 is primarily used in research settings as a potent inhibitor of Cdc25 phosphatases, which are crucial enzymes involved in cell cycle regulation. By inhibiting these enzymes, it helps in studying the mechanisms of cell cycle control and the potential for disrupting cancer cell proliferation. Its application is significant in the development of anticancer therapies, as it can induce cell cycle arrest and apoptosis in various cancer cell lines. Researchers also utilize NSC95397 to explore its effects on other phosphatases and its broader implications in cellular signaling pathways. This compound serves as a valuable tool in biochemical and pharmacological studies aimed at understanding and targeting abnormal cell growth in diseases like cancer.