Used primarily in pest control, especially in agriculture, as a powerful attractant for male Mediterranean fruit flies (Medflies). It is incorporated into traps to monitor and reduce Medfly populations, helping protect fruit crops such as citrus, apples, and stone fruits. Its high specificity makes it effective in integrated pest management programs, minimizing the need for broad-spectrum insecticides. Often used in combination with a killing agent in traps, Trimedlure supports quarantine efforts and helps prevent the spread of Medflies to non-infested areas.