Tylosin is widely used in veterinary medicine as an antibiotic to treat infections in animals, particularly in livestock and poultry. It is effective against a range of bacterial infections, including respiratory and gastrointestinal diseases. In cattle, it is commonly used to treat pneumonia and mastitis. For pigs, it helps control swine dysentery and enzootic pneumonia. In poultry, it is used to combat chronic respiratory disease and infectious synovitis.

Additionally, tylosin is sometimes added to animal feed as a growth promoter, helping to improve feed efficiency and weight gain in livestock. It is also used in aquaculture to prevent and treat bacterial infections in fish.

In beekeeping, tylosin is applied to control American foulbrood, a bacterial disease that affects honeybee larvae. Its use in various fields underscores its importance in maintaining animal health and productivity.