Estradiol hemihydrate is primarily used in hormone replacement therapy (HRT) to alleviate symptoms of menopause, such as hot flashes, vaginal dryness, and mood swings. It helps replenish estrogen levels in women experiencing natural or surgical menopause. Additionally, it is employed in the treatment of hypoestrogenism caused by hypogonadism, primary ovarian failure, or after oophorectomy. In some cases, it is used to prevent osteoporosis in postmenopausal women who are at high risk of fractures. It is also utilized in the palliative treatment of prostate cancer in men and in assisted reproductive technologies to support follicular development and endometrial preparation. Beyond medical applications, it is employed in veterinary medicine to manage hormonal imbalances in animals. Its efficacy in regulating estrogen-dependent processes makes it a critical component in various therapeutic and preventive treatments.