Oxytetracycline is widely used in both human and veterinary medicine as an antibiotic to treat a variety of bacterial infections. In humans, it is effective against respiratory tract infections, urinary tract infections, and skin conditions like acne. It is also used to manage infections caused by rickettsiae, mycoplasma, and chlamydia.

In agriculture, oxytetracycline is commonly applied to control bacterial diseases in livestock, including cattle, pigs, and poultry. It helps treat infections such as pneumonia, mastitis, and enteritis. Additionally, it is used in aquaculture to prevent and treat bacterial infections in fish and shellfish.

In horticulture, oxytetracycline is sometimes employed to combat bacterial diseases in plants, such as fire blight in fruit trees. Its broad-spectrum antibacterial properties make it a versatile tool in managing infections across diverse fields.