Brij-35 is widely used as a nonionic surfactant in various industries. In biochemistry and molecular biology, it is commonly employed to solubilize proteins and lipids, making it a key component in cell lysis buffers and electrophoresis applications. It helps maintain protein stability and prevents aggregation during experiments.

In the pharmaceutical industry, Brij-35 is utilized as an emulsifying agent in the formulation of creams, ointments, and other topical products. Its ability to improve the solubility of active ingredients enhances the effectiveness of drug delivery systems.

In analytical chemistry, Brij-35 is often added to mobile phases in liquid chromatography to improve the separation of hydrophobic compounds. It also acts as a wetting agent in various laboratory procedures, ensuring consistent and reliable results.

Additionally, Brij-35 finds applications in the cosmetics industry, where it is used in shampoos, conditioners, and skincare products to stabilize emulsions and enhance the texture and spreadability of formulations. Its mild nature makes it suitable for use in personal care products.