Tylosin is widely used in veterinary medicine as an antibiotic to treat bacterial infections in animals, especially in livestock such as cattle, swine, and poultry. It is effective against a range of gram-positive bacteria and some gram-negative organisms. Common applications include the treatment of respiratory infections, mastitis, and enteric diseases. Due to its antimicrobial properties, tylosin is also used as a growth promoter in animal feed to improve feed efficiency and weight gain. In addition, it plays a role in controlling certain mycoplasma infections, which are difficult to treat with other antibiotics. Its use is regulated in many countries to prevent antibiotic resistance and ensure food safety in animal-derived products.