O3-Deacetyl rocuronium bromide is a desacetylated derivative and potential metabolite or impurity of rocuronium bromide, a neuromuscular blocking agent. It is used primarily as an analytical reference standard for quality control, impurity detection, and stability testing in pharmaceutical formulations of rocuronium. This compound aids in ensuring the purity and safety of anesthesia drugs by identifying degradation products during manufacturing and storage processes.