Used primarily in diagnostic testing for adrenal gland function, particularly in the assessment of cortisol regulation. It inhibits cortisol synthesis by blocking the enzyme 11-beta-hydroxylase, leading to a rise in ACTH and precursor steroids, which helps evaluate the integrity of the hypothalamic-pituitary-adrenal axis. Commonly employed in the Metyrapone test to diagnose conditions like Cushing’s syndrome and adrenal insufficiency. Also investigated in research settings for studying stress responses and steroid-dependent diseases.