Mexenone is primarily used as an intermediate in the synthesis of vitamin K analogs and other biologically active compounds. It plays a key role in pharmaceutical manufacturing, especially in the production of anticoagulant agents and drugs targeting blood clotting disorders. Due to its quinone structure, mexenone participates in redox reactions, making it valuable in biochemical research and development of enzyme inhibitors. It is also utilized in studies related to mitochondrial function and cellular respiration, where it helps model electron transport chain behavior. In addition, mexenone finds application in the development of analytical reagents for detecting specific enzymes and in the formulation of certain diagnostic tools in clinical laboratories.