Penicillin G sodium is widely used in the treatment of various bacterial infections caused by susceptible microorganisms. It is particularly effective against gram-positive bacteria such as Streptococcus, Staphylococcus (non-penicillinase producing strains), and Clostridium species. It is commonly administered in serious infections like septicemia, endocarditis, and meningitis due to its rapid bactericidal action. It is also used in the management of respiratory tract infections, skin and soft tissue infections, and sexually transmitted infections such as syphilis. Due to its poor oral bioavailability, Penicillin G sodium is typically given intravenously or intramuscularly, making it suitable for hospital and clinical settings where parenteral administration is feasible. In veterinary medicine, it is used to treat bacterial infections in animals, especially in livestock. Its use is limited in outpatient settings due to the need for injection and frequent dosing, but it remains a critical antibiotic in acute care and for pathogens highly sensitive to penicillin.