Widely used in medical diagnostics, particularly in ophthalmology to detect corneal abrasions, ulcers, or foreign bodies. The solution is applied to the eye, where it stains damaged areas, making them visible under blue light. Commonly used in environmental studies to trace water flow and detect leaks in drainage systems, rivers, or sewage networks due to its high visibility in low concentrations. Employed in laboratory settings for cell viability assays; it helps distinguish live from dead cells based on membrane integrity—live cells exclude the dye, while dead cells take it up and fluoresce. Used in forensic science to enhance latent fingerprints on non-porous surfaces when exposed to UV light. Applied in industrial settings to test the integrity of pipelines and cooling systems by monitoring the presence and movement of the fluorescent dye.