Widely used as a fluorescent tracer in medical and biological applications, it helps visualize blood flow in the retina during angiography, aiding in the diagnosis of eye diseases like diabetic retinopathy and macular degeneration. It is also employed in flow cytometry and microscopy to label antibodies and proteins for detection. In environmental studies, it tracks water movement and detects leaks in drainage systems due to its high visibility in low concentrations. Industrial applications include testing the integrity of filters and detecting cracks in materials through fluorescent penetrant inspection. Its strong fluorescence under UV or blue light makes it valuable for educational demonstrations and forensic analysis.