Brij-35 is widely used as a non-ionic surfactant in laboratory and industrial applications. It is commonly employed in biological research for cell membrane permeabilization, allowing antibodies or other detection reagents to enter cells during immunostaining procedures. It is also used in enzyme-linked immunosorbent assays (ELISA) and Western blotting to reduce non-specific binding and improve signal clarity. In pharmaceutical formulations, Brij-35 enhances the solubility of poorly water-soluble drugs, aiding in the development of stable and effective dosage forms. Additionally, it finds use in cosmetic and personal care products as an emulsifier and solubilizing agent, helping to blend oil and water-based ingredients. Its low toxicity and good stability make it suitable for applications requiring biocompatibility.