Widely used as a non-ionic surfactant in laboratory and industrial applications, Brij-35 serves as an effective emulsifier and solubilizing agent. It is commonly employed in biochemical and molecular biology experiments, particularly in cell lysis buffers and protein extraction protocols due to its mild action on membrane structures. Frequently included in electrophoresis buffers and immunoassays such as ELISA and Western blotting, it helps reduce non-specific binding and improves assay consistency. In pharmaceutical formulations, Brij-35 enhances the solubility of hydrophobic compounds, aiding in drug delivery systems. It is also utilized in cosmetic and personal care products for its stabilizing and cleansing properties, especially in creams and lotions where low irritation is desired.