Used extensively in biochemical research as a fluorescent probe to study enzyme activity, particularly for detecting β-glucuronidase and other hydrolases. It serves as a substrate in assays to measure enzyme kinetics and inhibition. In medical diagnostics, it helps identify bacterial infections by detecting specific enzyme markers. Additionally, it is employed in cancer research to study hyaluronan synthesis inhibition, potentially impacting tumor growth and metastasis. Its fluorescence properties also make it useful in microscopy and cellular imaging techniques.