Primarily used in biochemical research as a fluorescent marker, it helps in studying cellular processes under a microscope. It is also employed in laboratory settings to test for the presence of certain enzymes, such as beta-glucosidase, by observing the hydrolysis reaction that produces esculetin, a compound that fluoresces under UV light. Additionally, it serves as a standard in chromatography for the identification and quantification of similar compounds in various samples. In medical research, it is utilized to investigate its potential anti-inflammatory and antioxidant properties, contributing to studies on treatments for conditions like chronic venous insufficiency.