Staurosporine is widely used in biomedical research as a potent inhibitor of protein kinases, including protein kinase C (PKC). It induces apoptosis in various cell types, making it a valuable tool for studying cell death mechanisms. Due to its ability to cross cell membranes easily, it is frequently applied in cell-based assays to modulate signaling pathways. It also serves as a reference compound in cancer research for evaluating the efficacy of new kinase inhibitors. Additionally, staurosporine is used in stem cell studies to promote differentiation or synchronize cell cycles. Its broad-spectrum kinase inhibition supports drug discovery and target validation in neurological and oncological fields.