Dolutegravir Impurity 16 is primarily used in the pharmaceutical industry as a reference standard in quality control processes. It plays a critical role in ensuring the purity and safety of dolutegravir, an antiretroviral medication used to treat HIV. By analyzing this impurity, manufacturers can identify and quantify its presence in drug formulations, ensuring compliance with regulatory standards. Additionally, it aids in the development and validation of analytical methods, contributing to the production of high-quality and effective medications for patients. Its use is essential in maintaining the consistency and reliability of the drug manufacturing process.