Used primarily as a reference standard in pharmaceutical testing and quality control processes, particularly in the analysis of nicotine-related products. It helps in identifying and quantifying impurities in nicotine-based medications, such as transdermal patches, gums, and e-liquids, ensuring product safety and regulatory compliance. Commonly employed in HPLC and GC-MS methods for accurate detection and separation of nicotine degradation products or synthesis by-products. Also utilized in research settings to study metabolic pathways and stability of nicotine compounds.