N-Nitroso Bisoprolol Solution is primarily used as a reference standard or impurity marker in pharmaceutical quality control and analytical testing. It plays a critical role in the detection and quantification of nitrosamine impurities in beta-blocker medications, particularly those containing bisoprolol. Due to the potential carcinogenicity of nitrosamines, regulatory agencies require strict monitoring of such impurities in drug products. This compound is used in high-performance liquid chromatography (HPLC) and mass spectrometry (MS) methods to validate testing procedures, ensure method accuracy, and support compliance with safety guidelines. It is not intended for therapeutic use but serves as an essential tool in ensuring the safety and purity of pharmaceutical formulations.