Used primarily as a reference standard in pharmaceutical research and quality control processes, especially in the analysis of linezolid and its related substances. It aids in the identification and quantification of impurities and degradation products during the manufacturing and stability testing of linezolid-based antibiotics. Commonly employed in high-performance liquid chromatography (HPLC) and mass spectrometry (MS) to ensure drug purity and compliance with regulatory requirements. Also utilized in metabolic studies to understand the breakdown pathways of oxazolidinone antibiotics in biological systems.