Dolutegravir Impurity 6 is primarily used in the pharmaceutical industry as a reference standard in the quality control and analytical testing of Dolutegravir, an antiretroviral medication. It helps in identifying and quantifying impurities during the drug manufacturing process, ensuring the final product meets regulatory standards for safety and efficacy. This impurity is critical for developing and validating analytical methods, such as HPLC or LC-MS, to monitor the purity and stability of Dolutegravir formulations. Its application is essential in maintaining consistency and compliance in the production of HIV treatment drugs.