Sofosbuvir Impurity C is primarily used in the pharmaceutical industry as a reference standard for quality control and analytical purposes. It plays a critical role in ensuring the purity and safety of Sofosbuvir, a key active pharmaceutical ingredient used in the treatment of hepatitis C virus (HCV) infections. By identifying and quantifying this impurity during the manufacturing process, researchers and manufacturers can maintain compliance with regulatory standards and ensure the efficacy of the final drug product. Additionally, it aids in the development and validation of analytical methods, contributing to the overall reliability of pharmaceutical testing protocols.