2'-O-Methylcytidine is widely used in the field of molecular biology and biochemistry, particularly in the synthesis of modified oligonucleotides. It plays a crucial role in the development of RNA-based therapeutics, such as antisense oligonucleotides and siRNA (small interfering RNA), where it enhances stability and resistance to nuclease degradation. This modification is also employed in the study of RNA structure and function, as it helps to investigate the role of specific nucleotides in RNA interactions and folding. Additionally, 2'-O-Methylcytidine is utilized in the design of probes for diagnostic applications, enabling the detection of specific RNA sequences in research and clinical settings. Furthermore, it is essential in the development of mRNA vaccines, improving RNA stability and reducing unwanted immune responses. Its incorporation into nucleic acids contributes to advancements in gene silencing, gene expression regulation, and the development of novel therapeutic strategies for various diseases.