Methyl palmitoleate is widely used in the cosmetic and personal care industry due to its emollient properties, which help to soften and smooth the skin. It is a common ingredient in moisturizers, lotions, and creams, providing hydration and improving skin texture.

In the field of biofuel production, it serves as a potential component in biodiesel formulations. Its favorable combustion properties and renewable nature make it a promising candidate for sustainable energy sources.

Additionally, it is utilized in research as a standard or reference compound in lipid analysis and metabolic studies, particularly in understanding fatty acid metabolism and its role in health and disease.

In the food industry, it can be used as a flavoring agent or additive, contributing to the sensory properties of certain products. Its applications extend to industrial lubricants, where it acts as a biodegradable and environmentally friendly alternative to traditional petroleum-based lubricants.