N-Nitroso-di-iso-propylamine is primarily encountered in research settings related to toxicology and carcinogenicity studies. It serves as a model compound for investigating the behavior of N-nitroso substances, which are known to exhibit potent mutagenic and carcinogenic properties. Due to its instability and health hazards, it is not used in commercial or industrial applications. Its main relevance lies in laboratory analysis for understanding nitrosamine formation, metabolic activation pathways, and DNA alkylation mechanisms. It may also be used in controlled studies to assess the effectiveness of anticancer agents or detoxification processes. Strict safety protocols are required when handling this compound because of its high toxicity and potential to cause long-term health effects.