Used in RNA research and nucleoside chemistry, 5-Methyluridine plays a key role in studying post-transcriptional modifications and stabilizing RNA structures. It is commonly incorporated into synthetic oligonucleotides to examine the effects of methylation on RNA function, including translation efficiency and ribosome binding. Its presence mimics naturally occurring modified nucleosides found in transfer and messenger RNAs, making it valuable for probing epigenetic regulation and RNA-protein interactions. Additionally, it serves as a building block in the development of modified RNAs for therapeutic applications, such as mRNA vaccines and antisense technologies, where enhanced stability and reduced immune activation are desired.