Defluoro Levofloxacin is primarily used as a reference standard or intermediate in pharmaceutical research and development. It plays a key role in the study of fluoroquinolone antibiotics, particularly in metabolic pathway analysis, where it helps identify degradation products and understand the stability of levofloxacin under various conditions. Due to its structural similarity to levofloxacin, it is also employed in bioanalytical assays to assess drug purity, detect impurities, and validate analytical methods. Additionally, it supports studies on antibiotic resistance mechanisms by serving as a model compound for evaluating bacterial enzyme interactions. Its use is mainly confined to laboratory and quality control settings rather than clinical treatment.