Used as a key intermediate in the synthesis of pH-sensitive fluorescent compounds, particularly for acid-base detection in cellular or biological environments. Its structure includes hydroxy and nitrogen groups capable of proton binding, which alters fluorescence properties according to pH levels. Widely applied in molecular biology research, such as monitoring intracellular processes, developing biosensors, and investigating protein or cell membrane behaviors. It can also be modified as a fluorescent label for high-resolution microscopy techniques. Solubility in water and polar solvents facilitates integration into aqueous systems.