Pseudomonic acid A is primarily used as an antibiotic, particularly effective against Gram-positive bacteria, including strains of Staphylococcus aureus. It is the active ingredient in the topical antibiotic mupirocin, which is widely applied to treat skin infections such as impetigo, folliculitis, and infected wounds. Its mechanism of action involves inhibiting bacterial isoleucyl-tRNA synthetase, which disrupts protein synthesis and prevents bacterial growth. Due to its localized application, it minimizes systemic side effects, making it a preferred choice for treating superficial infections. Additionally, it is used in nasal formulations to eradicate Staphylococcus aureus colonization in the nasal passages, particularly in healthcare settings to reduce the risk of surgical site infections. Its targeted action and low resistance development have cemented its role in dermatological and preventive healthcare.