Xylylazo Violet I is primarily used as a pH indicator in various analytical and laboratory applications. It is particularly effective in titrations where a clear color change is needed to determine the endpoint of the reaction. The compound exhibits a distinct color shift within a specific pH range, making it valuable for monitoring pH changes in chemical processes. Additionally, it is employed in the detection and quantification of metal ions, especially in complexometric titrations, due to its ability to form colored complexes with certain metals. This property is utilized in water quality testing and environmental analysis to assess the presence of metal contaminants. In biological research, it can be used as a staining agent to highlight specific cellular components or structures under microscopic examination. Its sensitivity to pH and metal ions makes it a versatile tool in both qualitative and quantitative chemical analyses.