Methyl Orange is widely used as a pH indicator in various chemical and biological applications. In titrations, it is particularly useful for determining the endpoint in acid-base reactions, as it changes color from red in acidic solutions (pH below 3.1) to yellow in alkaline solutions (pH above 4.4). This makes it a valuable tool in analytical chemistry for assessing the acidity or alkalinity of a solution.

In educational settings, it is commonly employed in laboratory experiments to teach students about pH and titration techniques. Additionally, it is used in environmental testing to monitor the pH levels of water bodies, ensuring they remain within safe and acceptable ranges.

In the textile industry, Methyl Orange serves as a dye for coloring fabrics, though its use in this area has diminished over time due to the development of more advanced dyes. Its role as a pH indicator, however, remains significant across scientific and industrial fields.