Used as a pH indicator in various analytical and laboratory applications, it changes color in response to pH levels, typically transitioning from yellow (acidic) to blue (alkaline) around pH 3.8 to 5.4. Commonly employed in titrations to determine the endpoint of acid-base reactions, it is particularly useful in biochemical assays and water quality testing. Additionally, it serves as a dye in microscopy to enhance the visibility of cellular structures. Its stability and distinct color change make it a reliable tool in both educational and research settings.