Used as a pH indicator in analytical chemistry, particularly in titrations involving weak acids and strong bases. It changes color in the pH range of 6.0 to 7.6, shifting from yellow to blue, making it suitable for detecting the endpoint near neutral pH. Commonly applied in medical tests, such as assessing amniotic fluid pH during labor to check for rupture of membranes. Also utilized in environmental testing and quality control in pharmaceuticals where precise neutralization detection is required.