Widely used as a pH indicator in analytical chemistry, this compound is especially effective in acid-base titrations due to its clear and distinct color change in the pH range of 8.0 to 9.6. It appears colorless in acidic solutions and shifts to a strong pink or red in basic conditions. Commonly employed in educational laboratories for demonstrating titration endpoints. Also utilized in environmental testing to assess the alkalinity of water samples. Its high sensitivity and reproducibility make it suitable for quality control in pharmaceutical and chemical manufacturing where precise pH monitoring is required. Additionally, it serves as an intermediate in the synthesis of other xanthene dyes and fluorescent probes used in biological staining and imaging applications.