Tetrabromophenol Blue sodium salt is widely used as a pH indicator in various analytical and laboratory applications. It is particularly effective in the pH range of 3.0 to 4.6, where it transitions from yellow to blue, making it useful for titrations and pH monitoring in chemical experiments. In biochemistry, it is employed in gel electrophoresis to track the progress of DNA or protein separation. Additionally, it serves as a dye in staining techniques to enhance the visibility of specific components under microscopic examination. Its stability and distinct color change also make it a valuable tool in educational settings for demonstrating acid-base reactions.