2,3-Dimethylbutane is a high-purity standard for gas chromatography (GC), exceeding 99.5% purity (GC), primarily used in analytical chemistry for calibrating instruments and identifying hydrocarbon compounds in complex mixtures.

Additionally, it is employed in fuel research, particularly for evaluating octane ratings due to its branched alkane structure. It serves as a reference substance in combustion studies to assess ignition behavior and engine performance in internal combustion engines. Its high volatility and flammability make it ideal for laboratory testing of fuel efficiency and emissions. It may also function as a solvent or diluent in specialized chemical processes requiring non-polar, inert hydrocarbons.