Rimsulfuron is primarily used as a selective herbicide in agriculture, especially in corn (maize) cultivation. It controls a broad spectrum of broadleaf weeds and some grassy weeds by inhibiting the enzyme acetolactate synthase (ALS), which is essential for amino acid synthesis in plants. This action stops weed growth at early stages, providing effective pre- and post-emergent control.

It is favored for its low application rates and rapid degradation in soil, reducing the risk of carryover to subsequent crops. Rimsulfuron is often combined with other herbicides, such as atrazine or mesotrione, to broaden the spectrum of weed control and help manage herbicide-resistant weed populations.

Due to its systemic nature, it is absorbed through roots and leaves and translocated throughout the plant, ensuring effective control even under varying environmental conditions. Its use supports conservation tillage systems by enabling efficient weed management with minimal soil disturbance.