Used primarily as a reference standard in analytical laboratories for the detection and quantification of organophosphorus compounds, especially in environmental and biological samples. It helps in calibrating instruments such as gas chromatographs and mass spectrometers for accurate measurement of pesticide metabolites and nerve agent byproducts. Due to its reactivity, it is also employed in research settings to study phosphorylation reactions and enzyme inhibition mechanisms. Strict handling protocols are followed due to its toxic and potentially hazardous nature.