p, p’-DDE is primarily used as a reference standard in environmental testing laboratories to detect and quantify pesticide residues, especially in studies related to organochlorine pesticide contamination. It serves as a key analytical standard in gas chromatography and mass spectrometry (GC-MS) for monitoring the breakdown products of DDT in soil, water, and biological samples. Due to its persistence in the environment and bioaccumulation in fatty tissues, p, p’-DDE is widely measured in wildlife and human tissue studies to assess long-term exposure to DDT and its ecological impact. It is also used in research investigating endocrine disruption and reproductive toxicity in animals, helping to understand the health effects of legacy pollutants.