This compound is primarily used in peptide synthesis as a protected form of serine. The tert-butyl (tBu) group protects the hydroxyl side chain, while the benzyl (Bzl) ester protects the carboxylic acid group. This protection ensures that specific reactions can occur at desired sites without interference from these functional groups. It is particularly valuable in solid-phase peptide synthesis (SPPS), where selective deprotection and coupling steps are essential for building complex peptide sequences. The hydrochloride (HCl) form enhances its stability and solubility, making it easier to handle in laboratory settings. Its application is critical in the production of pharmaceuticals, bioactive peptides, and research into protein structures and functions.