2-Fluoro-L-tyrosine hydrochloride is primarily used in biochemical and medical research as a labeled amino acid to study protein synthesis and metabolism. It serves as a tracer in positron emission tomography (PET) imaging, enabling the visualization and monitoring of cellular processes in vivo. This compound is particularly valuable in cancer research, where it helps track tumor growth and assess the efficacy of therapeutic interventions. Additionally, it is utilized in neuroscience to investigate neurotransmitter pathways and brain function. Its incorporation into proteins allows researchers to explore structural and functional aspects of biomolecules, contributing to advancements in drug development and molecular biology.