This chemical is primarily used in peptide synthesis as a protected derivative of (S)-2-methylaspartic acid. It serves as a building block for creating complex peptides, ensuring specific modified amino acids are incorporated correctly during solid-phase peptide synthesis (SPPS). The fluorenylmethoxycarbonyl (Fmoc) group acts as a protective group for the α-amine functionality, preventing unwanted reactions during the synthesis process. The tert-butoxy group, as part of the tert-butyl ester, protects the side-chain carboxylic acid moiety, allowing selective deprotection and activation when needed. This compound is particularly valuable in the production of peptides for pharmaceutical research, drug development, and biochemical studies, where precise control over peptide structure is critical. Its application is essential in synthesizing peptides with high purity and yield, making it a key reagent in modern peptide chemistry.