N-Fmoc-(S)-3-fluorohomophenylalanine is primarily used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS) methodologies. The Fmoc (fluorenylmethyloxycarbonyl) group serves as a protecting group for the amino group, ensuring selective deprotection during the synthesis process. The incorporation of the fluorine atom in the phenylalanine derivative enhances the peptide's stability and can influence its biological activity, making it valuable in the development of peptide-based drugs and biochemical probes. This compound is also utilized in research to study enzyme interactions and receptor binding, as the fluorine atom can act as a spectroscopic probe in NMR studies, providing insights into molecular structures and dynamics. Its application extends to the design of peptidomimetics, where it helps in mimicking the structure and function of natural peptides for therapeutic purposes.