Fmoc-3,4-dichloro-L-homophenylalanine is primarily used in peptide synthesis as a building block. It is particularly valuable in solid-phase peptide synthesis (SPPS) due to its Fmoc (fluorenylmethyloxycarbonyl) protecting group, which can be easily removed under mild basic conditions. The compound is often employed in the development of peptide-based drugs and bioactive peptides, where the presence of the dichloro-substituted phenylalanine moiety can enhance binding affinity and stability. It is also utilized in research to study peptide-protein interactions and to design peptide mimics for therapeutic applications, such as enzyme inhibitors or receptor modulators. Additionally, its unique structure makes it useful in the synthesis of complex peptides for use in biochemical and pharmacological studies.