Used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS), where it serves as a protected amino acid derivative. The Fmoc group provides temporary protection for the amino group, while the tert-butyl ester (OtBu) protects the carboxyl group, ensuring selective reactions during peptide chain assembly. This compound is especially valuable in constructing complex peptides with precise sequences, as it prevents unwanted side reactions. It is commonly employed in pharmaceutical research and development for creating peptide-based drugs, biomaterials, and bioactive compounds. Its stability and ease of deprotection make it a preferred choice in organic and medicinal chemistry laboratories.