This compound is primarily used in peptide synthesis as a protected form of glutamic acid. The tert-butyl (OtBu) group acts as a protective group for the side chain carboxyl functionality, while the benzyl (OBzl) group protects the alpha-carboxyl group, preventing unwanted reactions during the peptide chain assembly. The alpha-amino group is unprotected (free) but provided as the hydrochloride (HCl) salt for enhanced stability and solubility. It is particularly useful in solid-phase peptide synthesis (SPPS) and solution-phase methods where selective deprotection is required to build complex peptides. The HCl form facilitates handling and use in coupling reactions with various organic solvents. This specialty chemical intermediate is essential in the production of peptides for research, pharmaceuticals, and biotechnology applications, including the development of therapeutic peptides and peptide-based drugs.