This compound is primarily used in peptide synthesis as a protected amino acid derivative. It serves as a building block for constructing peptides with specific sequences, particularly in solid-phase peptide synthesis (SPPS). The fluorenylmethoxycarbonyl (Fmoc) group acts as a protective group for the amine functionality, ensuring selective reactions during peptide chain elongation. The nitro group on the phenyl ring can be utilized for further chemical modifications or as a handle for conjugation in bioconjugation chemistry. Its application is significant in pharmaceutical research, where it aids in the development of peptide-based drugs, bioactive peptides, and peptidomimetics. Additionally, it is employed in the study of enzyme-substrate interactions and protein engineering.