This compound is a protected derivative of (2S,4R)-4-aminoproline, primarily utilized in peptide synthesis as a building block for constructing complex peptides. It ensures specific amino acids are incorporated at desired positions without unwanted side reactions. The fluorenylmethoxycarbonyl (Fmoc) group protects the pyrrolidine nitrogen (serving as the alpha-amino equivalent), while the tert-butoxycarbonyl (Boc) group protects the 4-amino side chain functionality. This dual protection strategy enables selective deprotection during sequential peptide assembly, making it valuable in solid-phase peptide synthesis (SPPS). Its applications are particularly significant in pharmaceutical research and the development of peptide-based drugs, where precise control over peptide structure is critical.